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Hemolytic activity of LyeTxI and LyeTxI-b. Lamb erythrocytes suspended in PBS were incubated for 1 h with increasing concentrations from 0.35 to 44.8 μmol.L-1 and 0.36 to 46.8 μmol.L-1, of (A) LyeTxI and (B) LyeTxI-b, respectively. The control of 100% of cell lysis was considered by hemoglobin release in the presence of Triton X-100 (1% by volume). Hemoglobin release was measured at 405 nm. ED50: LyeTxI = 42.54 μmol.L-1 while LyeTxI-b at 46.8 μmol.L-1 had 46.83% of hemolysis.
