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. 2018 Apr 6;11:100. doi: 10.3389/fnmol.2018.00100

Figure 2.

Figure 2

Decreased expression levels of enkephalin (ENK) and delta opioid receptors (DOPr) mRNA are associated with vulnerability to social stress. (A) Schematic overview of amygdala region (Bregma −1.94 mm), Image credit: Franklin and Paxinos, 2nd edition. (B) ENK mRNA are decreased in the basolateral nucleus of amygdala (BLA) of vulnerable mice after RSD. (C) Magnified view of representative autoradiographs showing the amygdalar region selected (Bregma −1.94 mm) for ENK mRNA quantification by radioactive in situ hybridization. Scale bar = 0.5 mm. (D) Schematic overview of Hippocampus (HPC) for quantification in dorsal (Bregma −1.82 mm), central (Bregma −2.54 mm) and ventral HPC (Bregma −3.08 mm), and subiculum region (Bregma −3.52 mm), Image credit: Franklin and Paxinos, 2nd edition. (E) DOPr mRNA are decreased in the oriens layer (Or) of CA1 HPC in vulnerable mice after RSD. (F) Magnified view of representative autoradiographs showing the ventral HPC region selected (Bregma −3.08 mm) for DOPr mRNA quantification by radioactive in situ hybridization. Scale bar = 0.5 mm. For (B,E) One-way ANOVA, Bonferroni post hoc analysis, N(C) = 11, N(R) = 7, N(V) = 13, *p < 0.05, **p < 0.01. Error bars are mean ± SEM. The nomenclature used is from the Mouse brain atlas, Paxinos and Franklin, 2nd edition. BMP, posterior basomedial nucleus of amygdala; CeA, central amygdala; LA, lateral amygdala; BLA, basolateral amygdala; Pir, piriform cortex; CA1/CA3, regions in HPC; DG, dentate gyrus; Py, stratum pyramidale; Rad, stratum radiatum; Or, stratum oriens.