Table 2.
Descriptive statistics obtained by BestKeeper analysis.
| Gene | miR-21 | miR-16 | miR-148b | miR-92a | miR-34a | miR-191 | miR-152 | let-7a | miR-24 | miR-103a | BKI (n = 10) | BKI (n = 7) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| N | 57 | 57 | 57 | 57 | 57 | 57 | 57 | 57 | 57 | 57 | 57 | 57 |
| GM [Ct] | 22.733 | 23.492 | 29.789 | 26.157 | 27.999 | 27.741 | 29.281 | 24.124 | 25.017 | 26.058 | 26.138 | 26.573 |
| AM [Ct] | 22.786 | 23.543 | 29.825 | 26.182 | 28.026 | 27.762 | 29.311 | 24.170 | 25.059 | 26.102 | 26.165 | 26.598 |
| Min [Ct] | 18.592 | 19.075 | 26.276 | 22.905 | 25.543 | 24.954 | 27.126 | 21.330 | 22.417 | 23.362 | 23.983 | 24.757 |
| Max [Ct] | 27.486 | 29.219 | 34.338 | 28.479 | 31.928 | 30.182 | 33.021 | 30.964 | 30.615 | 32.121 | 30.087 | 30.484 |
| SD [±Ct] | 1.197 | 1.074 | 1.099 | 0.846 | 0.981 | 0.831 | 1.019 | 1.035 | 1.020 | 1.005 | 0.869 | 0.821 |
| CV [%Ct] | 5.255 | 4.562 | 3.685 | 3.231 | 3.500 | 2.994 | 3.478 | 4.280 | 4.070 | 3.852 | 3.320 | 3.086 |
| r with BKI (n = 10) | 0.830* | 0.863* | 0.914* | 0.751* | 0.785* | 0.820* | 0.842* | 0.899* | 0.929* | 0.961* | — | — |
| r with BKI (n = 7) | — | — | — | 0.745* | 0.780* | 0.801* | 0.854* | 0.923* | 0.942* | 0.963* | — | — |
| Ranking | 10 | 9 | 8 | 7 | 6 | 5 | 4 | 3 | 2 | 1 | — | — |
AM: arithmetic; BKI: BestKeeper Index; Ct: threshold cycle value; CV: coefficient of variation; GM: geometric; Max: maximum value; Min: minimum value; N: number of available samples; n: number of genes; r: Pearson correlation coefficient; SD: standard deviation; *p ≤ 0.001, P-value associated with the Pearson correlation coefficient. BKI was calculated for 10 genes and for seven genes, excluding the gene with the highest standard deviation.