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. 2018 Apr 12;8:5925. doi: 10.1038/s41598-018-24417-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Structure of the TFO probes and the TFT cassettes containing constructs: (a) TFO probe structure: the specificity head (underlined) is formed by a 22-mer oligonucleotide composed of a mixture of LNA and DNA residues. A psoralen residue is grafted at the 5′-end of the oligonucleotide. Its 3′-end is modified with a desthiobiotin residue attached to spacer composed of a linear chain of 124 atoms. (b) Construction of plasmids containing a Triplex Forming Tag (TFT) cassette: pAS03 is derived from pcDNA3.1(+)CAT. pAS03.1 is derived from pAS03 by inserting a TFT cassette containing two different TFO-target sites (TFT-1 and TFT-2). pAS03.2 is derived from pAS03.1 by inserting an additional TFT cassette. pAS04 is derived from pAS03.2 by inserting a third TFT cassette. pUC ori: high copy number origin in E. coli. SV40 ori: replication origin in primate cells expressing SV40 large T antigen. Ap: ampicillin resistance gene. Neo: neomycin resistance gene. CAT: chloramphenicol resistance gene. P_CMV: human cytomegalovirus immediate-early promoter/enhancer.