Fig. 2.

Ciliogenesis is reduced and cilia are bulged in Dzip1l mutant. (A) Reduced cilia number in Dzip1l mutants. Neural tube and limb bud sections and pMEFs of wt and Dzip1l mutant embryos were stained for ciliary markers Arl13b and acetylated tubulin (ac-Tub). Average cilia number per field under 100× lens (n≥5 sections from two embryos for each genotype) or percentage of ciliated pMEFs (n≥100 cells) were quantified and are shown to the right. NT, neural tube; LB, limb bud. *P≤0.00026, two-tailed Student's t-test. (B) Micrographs of SEM of neuroepithelia in wt and Dzip1l mutant neural tubes. Some cilia in Dzip1l mutants are bulged. Arrowheads point to cilia. The framed cilia are enlarged and shown to the right (n=2 experiments).