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. 2018 Apr 6;9:321. doi: 10.3389/fphar.2018.00321

FIGURE 1.

FIGURE 1

mRNA and fatty acid compositions of caffeine-treated worms. (A) 0.025, 0.05, 0.1, 0.2, 0.5 mg/mL caffeine all increased mRNA expression of fat-5, 0.1 mg/mL caffeine increased fat-5 expression approximately 2.5-fold; (B) 0.1 mg/mL caffeine did not affect mRNA expression of fat-6 or fat-7; (C,D) Results of fatty acid contents by GC analysis. Treatment with 0.1 mg/mL caffeine elevated the 16:1/16:0 ratio (three repeats, p = 0.0054). No significant distinction was observed in the 18:1/18:0 ratio. It was analyzed using Student’s t-test. The data are described as mean ± SEM; ∗∗p < 0.01; ∗∗∗p < 0.001.