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. Author manuscript; available in PMC: 2018 Apr 13.
Published in final edited form as: Exp Parasitol. 2015 Mar 24;153:139–150. doi: 10.1016/j.exppara.2015.03.012

Figure 3. SG-sCD23 is an IgE decoy receptor.

Figure 3

A. IL-10 induced FcεRI α chain expression on THP-1 cells (10 ng/million cells); gray fill: isotype control; black line: anti-FcεRI. B. IgE is inhibited from binding to THP-1 cells in the presence of schistosome-treated cell-free supernatants (thick black line) compared to untreated supernatants (thick gray line). Recombinant SG-sCD23 also inhibits exogenous IgE from binding to THP-1 cells (thin black line; gray fill: isotype control). C. SG-sCD23 does not appear to bind CD21. Ramos B cells were treated with SEA or SWAP for 18 hrs. Supernatants were incubated with sCD21 polypeptide at 4°C for 2 hours and probed with anti-CD21 in Western blot (shown). Blots were stripped and re-probed with anti-CD23 and sCD23 molecular weight patterns are indicated by blot labels.