Table II.
Mn- and Cu/Zn-SOD activities of leaf extracts from transgenic plants of alfalfa clone N4-4-2 containing T-DNA of pEXSOD10 grown in greenhouse and field conditions
| Isozyme | Greenhouse
|
Field
|
||
|---|---|---|---|---|
| N4-4-2 | Transgenic | N4-4-2 | Transgenic | |
| units/mg protein | ||||
| No. of observations | 3 | 12 | 3 | 15 |
| Mn-SOD | 130 ± 80 | 150 ± 40 | 370 ± 78 | 200 ± 34 |
| Cytosol Cu/Zn-SOD | 180 ± 24 | 210 ± 12 | 560 ± 52 | 350 ± 25 |
| Chloroplast Cu/Zn-SOD | 330 ± 62 | 363 ± 31 | 200 ± 64 | 690 ± 36 |
Each plant was sampled as three separate extracts from greenhouse or field plants (blocks). Values for the transgenic plants are the mean ± se of four (greenhouse) or five (field) independent transgenic plants that were not significantly different at the 5% level of probability.