Fig. 4.

EGFP-tagged Ajuba family proteins colocalize with LATS1. (A) MCF10A cells from the indicated stable cell lines were plated at low density (15,000 cells/cm²) and cultured for 48 h in total. Transgene expression was induced with Dox for 24 h before fixation and then cells were stained for LATS1 (red) and DNA (Hoechst 33342, blue). (B) MDCKIIG cells from the indicated stable cell lines were plated at low density and treated as in A. Insets show higher magnification of the boxed regions (yellow dashes).