Table 2.
Differentially expressed proteins with a putative role in the catabolism of DPA and aniline (ANI) identified in the proteome of P. putida grown on DPA or ANI compared to cells grown on Succinate (Succ).
| Spot no. | Protein identificationa | Protein name | ANI/Succ | DPA/Succ | Gene locus codeb |
|---|---|---|---|---|---|
| 521 | Biphenyl dioxygenase subunit alpha | BphA1 | 0.52** | 0.48** | CBL13_3823 |
| 7513 | Biphenyl dioxygenase subunit alpha | BphA1 | 0.98 | 0.27*** | CBL13_3823 |
| 7612 | Biphenyl dioxygenase subunit alpha | BphA1 | 0.78 | 0.26*** | CBL13_3823 |
| 7613 | Biphenyl dioxygenase subunit alpha | BphA1 | 0.72 | 0.28** | CBL13_3823 |
| 7616 | Biphenyl dioxygenase subunit alpha | BphA1 | 0.43** | 0.44*** | CBL13_3823 |
| 8609 | Biphenyl dioxygenase subunit alpha | BphA1 | 0.54 | 0.26* | CBL13_3823 |
| 5409 | Biphenyl dioxygenase ferredoxin-NAD(+) reductase component | BphA4 | 2.39*** | 3.11*** | CBL13_3827 |
| 7413 | Biphenyl dioxygenase ferredoxin-NAD(+) reductase component | BphA4 | 32.85*** | 30.82*** | CBL13_3827 |
| 708 | Glutamine synthetase component of aniline dioxygenase | TdnQ | 2.28** | 1.48 | CBL13_5652 |
| 3608 | Glutamine synthetase component of aniline dioxygenase | TdnQ | 1.12 | 7.26*** | CBL13_5652 |
| 3714 | Glutamine synthetase component of aniline dioxygenase | TdnQ | 1.51** | 2.48*** | CBL13_5652 |
| 4112 | Glutamine amidotransferase component of aniline dioxygenase | TdnT | 8.55* | 1.82 | CBL13_5653 |
| 7001 | Aniline dioxygenase subunit beta | TdnA2 | 4.35* | 3.13* | CBL13_5655 |
| 7002 | Aniline dioxygenase subunit beta | TdnA2 | 6.99** | 4.72*** | CBL13_5655 |
| 7408 | Muconate cycloisomerase | CatB | 16.66*** | 16.91*** | CBL13_1890 |
| 7413 | Muconate cycloisomerase | CatB | 32.85*** | 30.82*** | CBL13_1890 |
| 7415 | Muconate cycloisomerase | CatB | 7.10*** | 12.28*** | CBL13_1890 |
| 1108 | 3-oxoadipate enol-lactonase | PcaD5 | 29.56*** | 17.30* | CBL13_3870 |
| 5109 | 3-oxoadipate CoA-transferase subunit A | PcaI2 | 43.84* | 43.02* | CBL13_2043 |
| 1109 | 3-oxoadipate CoA-transferase subunit B | PcaJ2 | 23.63** | 27.54** | CBL13_2044 |
| 7308 | Beta-ketoadipyl-CoA thiolase | PcaF2 | 1.00 | 4.05** | CBL13_3873 |
| 7415 | Beta-ketoadipyl-CoA thiolase | PcaF2 | 7.10*** | 12.28*** | CBL13_3873 |
| 8509 | 3-carboxy-cis, cis-muconate cycloisomerase | PcaB | 34.1* | 25.48** | CBL13_3871 |
The spot intensity ratios of the ANI/Succ and DPA/Succ are shown as a measure of proteins differential expression (p-value < 0.05, < 0.01, < 0.001 are indicated by *, **, and *** respectively).
a
Protein annotation based on homology with the translated genome of P. putida DPA1.
b
The locus number of the gene which showed the highest homology with the sequenced protein spot.