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. 2018 Feb 19;37(8):e97349. doi: 10.15252/embj.201797349

Figure EV2. Cholesterol depletion negatively shifts the “fast” inactivation voltage dependency of Nav1.9.

Figure EV2

  1. Families of Nav1.9 current traces recorded after 10‐min treatment with 20 mM MβCD. The test pulse to −30 mV was preceded by a family of depolarizing pulses ranging from −80 to 35 mV for 100 ms, while the cell was held at −120 mV.
  2. Fast inactivation curves of Nav1.9 current in control DRG neurons (n = 3) and in neurons treated with 20 mM MβCD (n = 3) or 2 U/ml ChOxi (n = 2) for 10 min. Curves were fitted by using single Boltzmann equations, yielding V 0.5 values of −25.17 ± 1.14, −34.8 ± 1.1, and −41.54 ± 0.38 mV, respectively. Values are shown as mean ± standard error of the mean (SEM).