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. 2018 Feb 19;37(8):e97349. doi: 10.15252/embj.201797349

Figure EV4. Activation of Nav1.9 by GTPγS, a non‐hydrolyzable G protein‐activating analog of GTP, is prevented by soluble cholesterol.

Figure EV4

  1. Representative current traces evoked by 100‐ms depolarizing voltage steps from −80 to +20 mV (Δ5 mV, Vh = −100 mV). Nav1.9 currents were recorded 10 min after achieving whole‐cell recording configuration with patch pipette solution containing 400 μM of GTPγS. In the right panel, DRG neurons were pre‐incubated prior recording with MβCD‐chol (20 mM) for 10 min.
  2. Currents evoked at −55, −50, and −40 mV from (A) are illustrated.
  3. Corresponding activation curves of Nav1.9 current fitted with a single Boltzmann equation. Values for V 0.5 of activation are −27.4 ± 0.7 mV (n = 7, green line) and −49.2 ± 0.8 mV (n = 8, red line) with and without MβCD‐chol, respectively. Values are shown as mean ± standard error of the mean (SEM).