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. 2018 Feb 19;37(8):e97349. doi: 10.15252/embj.201797349

Figure EV5. Cholesterol gels do not impair cell viability, skin structure, and mechanical threshold.

Figure EV5

  1. Cell viability of reconstructed human epidermis measured with an MTT assay 48 h after exposure to PBS (non‐toxic control, n = 3), 5% SDS (toxic control, n = 3), HEC gel containing no cholesterol (0 mM, n = 3), and HEC gels containing soluble cholesterol at the concentration of 5.6 or 28 mM (n = 3 each).
  2. Hematoxylin–eosin staining of standardized skin biopsy specimen from paw treated for 1.5 h with an HEC gel containing 28 mM of cholesterol. sc: stratum corneum; e: epidermis; d: dermis.
  3. Mechanical withdrawal threshold of mice treated for 1.5 h with an HEC gel containing 28 mM of cholesterol (n = 9).
Data information: All values are shown as mean ± standard error of the mean (SEM).