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. 2018 Feb 19;37(8):e97349. doi: 10.15252/embj.201797349

Figure 7. Cholesterol delivery prevents the modulation of Nav1.9 currents by inflammatory mediators.

Figure 7

  1. Representative current traces evoked by 100‐ms depolarizing voltage steps ranged from −80 to +50 mV (Δ 5 mV, Vh = −100 mV) of DRG neurons treated with inflammatory cocktail, inflammatory cocktail plus MβCD‐chol, or MβCD‐chol alone. DRG neurons were pre‐incubated for 15 min at 37°C with DMEM alone or supplemented with 20 mM of MβCD‐chol; when used, the inflammatory cocktail was added to extracellular solution at room temperature (RT).
  2. Nav1.9 currents evoked at −40 and −35 mV (gray and black traces, respectively) extracted for clarity's sake from (A).
  3. Corresponding current–voltage relationships of Nav1.9 currents treated with an inflammatory cocktail, inflammatory cocktail plus MβCD‐chol, or MβCD‐chol alone.
  4. Mean activation curves of Nav1.9 currents recorded in the different conditions fitted with single Boltzmann equations. Values for V 0.5 of activation are −27.3 ± 0.2 mV in control conditions (n = 5, black dashed line, same as Fig 4B), −28.9 ± 1.6 mV for MβCD‐chol (n = 8, orange line), −34.5 ± 2 mV for inflammatory cocktail (n = 7, red line), and −26.7 ± 0.7 mV for MβCD‐chol + inflammatory cocktail (n = 11, green line). Values are shown as mean ± standard error of the mean (SEM).