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. 2018 Mar 6;37(8):e97902. doi: 10.15252/embj.201797902

Figure EV1. Structural analysis & size‐exclusion chromatography of the Aurora‐A/TACC3 complex.

Figure EV1

  1. Size‐exclusion chromatographs of Aurora‐A and TACC3 alone and in complex on a Superdex 200 16/600 column (GE Healthcare).
  2. Electron density map of the Aurora‐A/TACC3 complex. Aurora‐A is coloured cyan. TACC3 is coloured orange. The 2mFo‐DFc electron density map is shown as a wire mesh contoured at 1.0 σ.
  3. Side chain HC chemical shift perturbations observed for 13C15N TACC3 519–540 on the addition of Aurora‐AKD D274N.
  4. Small section showing mainly methyl group resonances from a 13C‐HSQC spectrum of TACC3 519–540. The spectrum for TACC3 519–540 is shown in blue. The spectrum collected on the addition of Aurora‐AKD D274N is coloured red.