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. 2018 Mar 7;37(8):e97825. doi: 10.15252/embj.201797825

Figure 2. Absence of a disulfide bond in the periplasmic loop of its CSS domain results in processing of PdeC to a 33‐kD C‐terminal fragment.

Figure 2

  1. Derivatives of strain W3110 containing the indicated plasmids were grown in LB medium, and samples were taken during post‐exponential growth and from overnight (“on”) cultures. PdeC variants (containing C‐terminal His6 tags) produced from the indicated plasmids were detected by immunoblotting.
  2. Strain W3110 containing the PdeCwt plasmid was grown to an OD578 of 1.2 and 6.5 mM DTT was added where indicated, with samples for immunoblotting taken at the times indicated. As a reference for the 33‐kD proteolytic fragment of PdeC, a sample obtained from the PdeCASS plasmid strain after overnight growth was included (last lane).
  3. Derivatives of strain W3110 carrying the indicated mutations in the different dsb genes and containing the plasmids expressing either PdeCwt or PdeCASS were grown in LB medium. Samples were taken during post‐exponential growth and from overnight cultures and PdeC variants expressed were visualized by immunoblotting.

Source data are available online for this figure.