Figure 3. The periplasmic proteases DegP and DegQ are responsible for proteolytic processing of PdeC, but processing is not a prerequisite for PDE activity of PdeC.

1. Derivatives of strain W3110 carrying single or double mutations in degP and degQ and containing the plasmids expressing either PdeC^wt or PdeC^ASS were grown in LB. Samples were taken during post‐exponential growth (pe) or overnight (on), and PdeC variants expressed were visualized by immunoblotting. The dsbA mutant was included to show the position of the 33‐kD processed fragment of PdeC.
2. Macrocolony morphology of the degP degQ derivative of AR3110 carrying the indicated plasmids indicates high PDE activity of PdeC^ASS; that is, processing to the 33‐kD fragment is not required for high PDE activity of PdeC^ASS.

Source data are available online for this figure.