Figure 5. Chromosomally encoded PdeC shows redox control and DegP/DegQ‐mediated proteolysis and a second CSS domain PDE, PdeB, is controlled similarly.

1. Derivatives of strain W3110 in which the chromosomal pdeC and pdeB genes carry 3′‐inserts encoding Flag tags were grown in LB medium. DTT treatment of the cultures (left panels) was as described in the legend to Fig 2. Strains shown in the right panels also carried the indicated mutations. Samples were taken during post‐exponential phase (at an OD₅₇₈ of 3–3.5) and from overnight cultures and subject to immunoblot analysis with Flag tag antibodies.
2. Macrocolonies of Escherichia coli K‐12 strain AR3110 expressing the indicated PdeB variants under control of the tac promoter from the low copy number plasmid pCAB18 were grown on salt‐free LB (LBnoS) with the matrix dye Congo red (CR) for 5 days at 28°C.

Source data are available online for this figure.