Figure EV1. The PI3K pathway was active in ASC but not QSC .

1. Freshly isolated single myofibers from 2‐month‐old wild‐type mice were fixed either immediately or 24 h after culturing.
2. TA muscles from uninjured or cardiotoxin (CTX)‐injured mice (2.5 days post‐injury) were dissected, cryosectioned, and fixed. Myofibers from (A) and TA muscle sections from (B) were then subjected to immunostaining for p‐S6 (green) together with Pax7 (A) and YFP (B) (red), respectively. The nuclei were counterstained with DAPI (blue). Arrowheads: YFP⁺ MuSCs. Scale bars: 25 μm.