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. 2018 Mar 26;37(8):e98239. doi: 10.15252/embj.201798239

Figure 2. Ablation of p110α in adult MuSCs from uninjured muscles reduced Pax7 protein levels without obviously affecting the MuSC number.

Figure 2

  1. Adult control and iKO mice (n = 3) were treated with Tmx as described in Fig 1. Seven days after the last injection of Tmx, mice were sacrificed and TA muscle sections were immunostained for Pax7 (red). The nuclei were counterstained by DAPI (blue).
  2. Quantification of Pax7+ MuSCs per field of TA muscle sections from (A) by examining multiple sections from three pairs of mice.
  3. Freshly isolated single myofibers (n > 20) from three pairs of Ctrl or iKO mice were immediately fixed and immunostained for Pax7.
  4. Top: representative FACS plots showing the percentage of Vcam1+ MuSCs (boxed areas) among the CD31−CD45−Sca1− cell populations from uninjured muscles of the Ctrl and iKO mice (n = 3 in each group). Bottom: Sorted YFP+ MuSCs were immediately fixed and immunostained for Pax7 (green). The nuclei were counterstained by DAPI (blue).
  5. Quantification of YFP+ MuSCs in whole hindlimb muscles by FACS from the Ctrl and iKO mice (n = 5).
  6. FACS‐isolated MuSCs from iKO mice (without Tmx) were cultured for 2 days followed by 2 days of 4‐OHT treatment. Whole‐cell lysate was subjected to Western blotting.
Data information: Scale bars: 50 μm. In (B–E), the data are presented as mean ± s.d. ***P < 0.001; unpaired Student's t‐test.