Figure EV3. Reduction in Pax7 protein in p110α‐null MuSCs was mediated by autophagy.

• A, B
  FACS‐isolated MuSCs from iKO mice (without Tmx) were cultured for 2 days followed by 1 day of 4‐OHT treatment. Then, the cells were either treated with DMSO or LY294002 (10 μM) in (A) or transfected with siRNAs in (B) as indicated and grown in the presence of 4‐OHT for another day before harvest. Whole‐cell lysates were subjected to Western blotting.