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. 2018 Mar 26;37(8):e98239. doi: 10.15252/embj.201798239

Figure EV4. p110α‐null MuSCs were arrested in the G0 state.

Figure EV4

  1. FACS‐isolated MuSCs from the tamoxifen (Tmx)‐treated Ctrl and iKO mice were cultured for 36 h followed by a 4‐h EdU pulse. Cells were fixed and stained for EdU (green) and DAPI (blue).
  2. Quantification of the EdU+ MuSCs from (A) by counting ˜600 MuSCs from triplicate wells.
  3. Histograms showing the mean FSC‐A values of MuSCs freshly isolated from the Ctrl and iKO mice.
  4. Freshly isolated single myofibers (n > 20) from the Ctrl and iKO mice (n = 3 in each group) were immediately fixed and stained for CD34 (red), YFP (green), and DAPI (blue). A representative image is shown.
  5. Quantification of the areas of MuSCs from (D) based on CD34 staining. More than 100 MuSCs were calculated for each group of mice.
  6. FACS‐isolated MuSCs from the Tmx‐treated Ctrl and iKO mice were cultured for 36 h followed by staining for MyoD (green) and DAPI (blue).
  7. Quantification of the MyoD+ ASCs from (F) by counting ˜600 ASCs in triplicate wells. Scale bars: 25 μm. In (B, E, G), the data are presented as mean ± s.d. ***P < 0.001.