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. 2018 Mar 26;37(8):e98239. doi: 10.15252/embj.201798239

Figure EV5. mTORC2 was not required for the cell cycle re‐entry of MuSCs from idKO mice.

Figure EV5

  1. FACS‐isolated MuSCs from iKO and idKO mice were transfected with siRNAs as indicated in the presence of EdU for 36 h followed by cell fixation and staining for EdU (green) and DAPI (blue).
  2. Quantification of the EdU+ MuSCs in (A) by counting ˜600 MuSCs in triplicate wells.
  3. Triplicate C2C12 cells were transfected with indicated siRNAs for 24 h. Total RNAs were extracted. The mRNA levels of Rictor were measured by RT–qPCR. Scale bar: 50 μm. In (B, C), the data are presented as mean ± s.d. ***P < 0.001.