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. 2018 Mar 26;37(8):e98239. doi: 10.15252/embj.201798239

Figure EV8. MuSCs from ip110αca mice proliferated and differentiated faster.

Figure EV8

  1. TA muscle sections from the Ctrl and ip110αca mice as described in Fig 7A were stained for EdU (white), YFP (red), laminin (green), and DAPI (blue). Arrowheads: YFP+/EdU−, YFP−/EdU+, or YFP+/EdU+ cells.
  2. TA muscle sections as described above were stained for EdU (green) and dystrophin (red).
  3. Quantification of the EdU+ cells underneath the dystrophin+ sarcolemma in (B). Three pairs of mice were examined including five TA muscle sections per mouse and three microscopic fields per section.
  4. FACS‐isolated MuSCs from the Ctrl or ip110αca mice were cultured for 24 h followed by a 4‐h EdU pulse.
  5. Quantification of the EdU+ ASCs in (D) by counting ˜600 ASCs in triplicate wells.
  6. FACS‐isolated MuSCs from the Ctrl or ip110αca mice were cultured for 3 days followed by cell fixation and staining for MHC (red), p‐S6 (green), and DAPI (blue).
  7. Quantification of the MHC+ cells in (F) by counting ˜600 nuclei in triplicate wells. Scale bars: 25 μm. In (C, E, G), the data are presented as mean ± s.d. **P < 0.01; ***P < 0.001.