Fig. 5. pH-Dependent fluorescence observed for NBC-labelled PCysMA and POEGMA brushes using a confocal laser scanning microscope (CLSM) with spectral resolution capability. (a) Microscope images excited at 543 nm with 10 nm resolution. (b) Top: pH-dependent fluorescence spectra based on CLSM images of NBC-labelled POEGMA brushes (approximate dry brush thickness = 60 nm). Bottom: pH-dependent fluorescence spectra based on CLSM images of NBC-labelled PCysMA brushes (approximate dry brush thickness = 10 nm). (c) Ratiometric fluorescence vs. pH plot obtained for these PCysMA and POEGMA brushes.
