Fig. 2. Identification of asperphenamate (apm) biosynthetic gene cluster. (A) HPLC analysis of crude extracts from wild type (WT) strain (i) and apm deletion mutants (ii, iii): apmA and apmB deletion strains eliminated 1 production; ΔapmB accumulated 3 production (iii); feeding compound 3 into the apmA deletion strain restored 1 production (iv); traces v, vi show the pure compounds 1, 3. (B) Organization of apm biosynthetic genes in P. brevicompactum and their homologues in A. terreus and A. aculeatus.