Fig. 4. ApmB utilizes linear dipeptidyl precursor to synthesize asperphenamate. (A) HPLC analysis of metabolites produced from ApmB expressing-strain with feeding 13C,15N-2 and 3. 13C,15N-1 was obtained. (B) LC-MS confirmation of labeled 13C,15N-2 integration into final product of 1. (C) ApmB-catalysed reactions by using 13C,15N-2 and 3 as substrates. Labelled 13C,15N-1 was characterised by NMR and MS analysis.
