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Anti-neurofilament immunostaining of (a) the control nerves (b to g) and all groups, 60 days after surgery. h Quantification of the integrated density of pixels in the experimental groups relative to the control group presented in percentage (%) and calculated using Image J software. Statistically, there is no significant difference among the experimental groups. Scale bar: 50 μm. N: neurorrhaphy, F: heterologous fibrin sealant, D: doxycycline, T: transgenic hESCs
