Fig. 5.

Functionality of Rpf-mCherry/EGFP fusions: nutritional shift-down assay. The assay was performed as detailed in Methods. a Rpf-mCherry fusions are functional: their overproduction stimulates the growth of stationary cells subjected to nutritional shift-down much faster than the overproduction of mCherry alone. Growth curves were made with data collected from 3 biological replicates. Error bars indicate standard error of the mean. b Rpf-EGFP fusions are not functional: their overproduction does not stimulate the growth of cells subjected to nutritional shift-down any better than the negative control overproducing EGFP. A representative growth curve (of at least 3 biological replicates) is shown for each strain