Fig. 6.

Induction of the growth of non platable cells by Rpf-mCherry producing strains after different stress conditions. The strains were subjected to three stress conditions and subsequently the potential for resuscitation of non platable cells was calculated for each strain as detailed in Methods and expressed as the Resuscitation Index (RI): log₁₀(MPN)-log₁₀(CFU). a Strains overproducing RpfD-mCherry and RpfE-mCherry show enhanced growth of non platable cells produced under pH 4.5, but this is not statistically significant. b The strain overproducing RpfE-mCherry enhances the growth of non platable cells produced under nutrient starvation to a greater extent, followed by RpfB-mCherry and RpfA-mCherry. c None of the strains overproducing Rpf-mCherry enhances the growth of non platable cells produced under 1 M NaCl. Three independent experiments were performed for each condition and strain. Error bars indicate standard error of the mean. Statistical differences in RI between strains were calculated using One Way Analysis of Variance followed by Bonferroni t-test. Cases in which differences were found to be statistically significant, are indicated by asterisks: ***P < 0.001; **P < 0.01; *P < 0.05