Table 1.
Aromatic halogenation catalyzed by different FDH or FDH-FR fusions.a
| Entry | FDH | Substrate | TTN | [FDH] (μM) | Yield (%) |
|---|---|---|---|---|---|
| 1 | RebH | 7 | 301 ± 2.3 | 1.5 | 90.3 |
| 2 | H-22-F | 7 | 128 ± 2.7 | 1.5 | 38.3 |
| 3 | H-16-F | 7 | 187 ± 11 | 1.5 | 56.2 |
| 4 | H-10-F | 7 | 182 ± 2.5 | 1.5 | 54.6 |
| 5 | H-16-Fre | 7 | 101 ± 1.5 | 1.5 | 30.3 |
| 6 | 3SS | 8 | 117 ± 0.01 | 1 | 23.4 |
| 7 | 3SS-16-F | 8 | 74 ± 4.65 | 1 | 14.8 |
| 8 | 1K | 9 | 382 ± 38 | 1 | 38.2 |
| 9 | 1K-16-F | 9 | 41 ± 11 | 1 | 4.1 |
| 10 | 10S | 10 | 11 ± 0.22 | 25 | 26.3 |
| 11 | 10S-16-F | 10 | 7 ± 0.02 | 25 | 17.3 |
a
0.5 mM substrate, 1.5-25 μM FDH, 9 U mL−1 GDH, 10-100 mM NaCl, 20 mM glucose, 100 μM NAD and FAD, 25 mM HEPES buffer pH = 7.4, 25 °C, 75 μL final reaction volume. 2.5 μM reductase was added to reactions that did not contain a fusion enzyme. Reactions were quenched with one volume MeOH. 0.5 mM phenol (for 7, 8, 10) or 0.5 mM benzoic acid (for 9) was added as an internal standard, and reactions were analyzed by HPLC.