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. Author manuscript; available in PMC: 2018 Apr 13.
Published in final edited form as: Vasc Med. 2017 Aug 19;22(5):363–369. doi: 10.1177/1358863X17722211

Figure 4.

Figure 4

KLF2/KLF4 fail to mediate transactivation of the mutant HBA promoter. (A) Schematic picture of the luciferase reporter driven by a fragment (953 bp, counting from the transcription start site) of the human HBA promoter. Four positions of consensus recognition sites for KLFs are indicated by white rectangles. (B and C) The mutant HBA promoter lacking four KLF-binding sites was co-expressed with either KLF2 (B) or KLF4 (C) (n=3–4 independent experiments). Gray boxes denote mutant KLF-binding sites on the HBA promoter. Data are presented as mean ± SEM values. **p < 0.01. KLF2, Kruppel-like factor 2 plasmid; KLF4, Kruppel-like factor 4 plasmid; HBA, hemoglobin subunit alpha; Luc, luciferase reporter; NS, not significant.