Skip to main content
. Author manuscript; available in PMC: 2018 Apr 13.
Published in final edited form as: Cancer Cell. 2015 Apr 13;27(4):516–532. doi: 10.1016/j.ccell.2015.03.006

Figure 2. Signaling and Oncogenic Role of Mutated STAT3.

Figure 2

(A) Immunoblotting from whole-cell lysates of reconstituted STAT3−/− MEF cells.

(B) Immunoblotting from whole-cell lysates of reconstituted human lung adenocarcinoma A549 cells.

(C) Immunoblotting from whole-cell lysates of reconstituted STAT3−/− MEF with WT or YF STAT3 constructs after human IL-6 stimulation and its neutralization with specific antibodies against human IL-6. The relative ratio pSTAT3/STAT3 (P-S/S) is indicated.

(D) Colony assay in reconstituted STAT3−/− MEF cells.

(E) Soft agar colony assay in reconstituted STAT3−/− MEF cells.

(F) Histology and phospho-STAT3 expression by immunohistochemistry in mice injected with reconstituted STAT3−/− MEF cells. The red scale bar represents 1.5 mm, the green scale bar represents 10 μm, and the black scale bar represents 20 μm.

See also Figure S2.