Skip to main content
. Author manuscript; available in PMC: 2018 Apr 13.
Published in final edited form as: J Am Chem Soc. 2017 Apr 10;139(15):5588–5595. doi: 10.1021/jacs.7b01791

Figure 3.

Figure 3

Application of new nucleophile probes for studying cellular sulfenylomes. (a) RKO whole cell lysates were prepared under nondenaturing conditions. Native lysate was divided into equal portions and incubated with one of five nucleophile probes (or vehicle) in separate reactions. Labeled proteins from each reaction were digested separately and the resulting peptides subjected to click reaction with UV-cleavable biotin-N3. Enrichment and photorelease afforded different probe-labeled peptides samples for proteomic analysis. (b) Protein Venn diagram of unique sulfenylated proteins. (c) Site Venn diagram of unique sulfenylated peptides.