Figure 1.

Conformational Change of FG-Nup214 upon Binding with CRM1 and RanGTP

(A) Scheme of FG-Nup214 construct and FG-Nup214¹¹⁷, labeling sites, and CRM1⋅RanGTP complex. Eight FG motifs (FG1–FG8) binding to CRM1 pockets are indicated. The nomenclature of binding regions is adapted from Port et al. (2015). F residues are only shown in the zoom-in region.

(B) S versus E_FRET histograms of 50 pM FG-Nup214 in the absence and presence of CRM1 and CRM1⋅RanGTP (from left to right, at 1 μM for both CRM1 and RanGTP concentrations). In the presence of CRM1, the E_FRET population (at S = 0.5) shows two populations corresponding to bound and unbound forms. In the presence of RanGTP, one distinct conformation with very low E_FRET is seen, suggesting a much more expanded conformation than in the unbound state. See also Figures S1 and S2.