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. Author manuscript; available in PMC: 2018 Apr 13.
Published in final edited form as: Nat Protoc. 2017 Sep 21;12(10):2169–2188. doi: 10.1038/nprot.2017.088

Figure 6. Flow cytometric analysis layout for human engraftment.

Figure 6

(a) Contaminating dead cells (PI+), mouse red blood cells (RBC, Ter119+) and cell doublets (low SSC-W and FSC-W) should be excluded prior to gating for human CD45/HLA-ABC double positive cells. (b) Transplanted and non-transplanted humanized ossicle bone marrow (BM), as well as murine BM (mBM) and murine peripheral blood (pB) should be analyzed by flow cytometry for the presence of human normal or leukemic CD45+HLA-ABC+ cells. Engrafted human cells should be defined by their expression of CD45 and HLA-ABC. These cells can be further gated for myeloid cells (CD45+HLA-ABC+CD33+) and B lymphoid cells (CD45+HLA-ABC+CD19+). Engrafted hematopoietic stem cells should result in bi-lineage engraftment (c), whereas myeloid leukemia engraftment should exclusively express CD33 and lack CD19 (d).