Extended Data Figure 9. Edited HSPCs from sickle cell patients differentiate into erythrocytes that express Glycophorin A.

CD34⁺ HSPCs derived from sickle cell patients were edited with HBB Cas9 RNP and either the corrective SNP donor or the cDNA donor. 4 days post-electroporation, cells edited with the cDNA donor were sorted for tNGFR⁺ cells. This population as well as the populations edited with the corrective SNP donor and Mock-electroporated cells were subjected to a 21-day erythrocyte differentiation protocol, followed by staining for Glycophorin A (GPA). All data points within experimental groups are derived from experiments in cells from different sickle cell patients, N=3 (Mock) and N=2 (SNP and cDNA donor).