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. Author manuscript; available in PMC: 2018 Apr 13.
Published in final edited form as: Cell Metab. 2016 Apr 12;23(4):712–724. doi: 10.1016/j.cmet.2016.03.004

Figure 1. Improved Lipid and Glucose Metabolism but No Changes in Ectopic Lipid Deposition in HFD-Fed AAV-B186-Expressing Mice.

Figure 1

C57BL/6JOlaHsd males transduced with AAVs and fed a HFD were used for the experiments.

(A–E) Comparison of (A) body weight, (B) food consumption, and fasting levels of (C) triglycerides, (D) glucose, and (E) insulin.

(F–H) IP-GTT (F), IP-ITT (G), and area under the curce (AUC) (H).

(I and J) Triglyceride concentration in the (I) liver and (J) heart.

(K) Visualization of lipid droplets in Plin5-stained heart sections.

(L) eWAT gene expression analysis by qPCR.

(M and N) Crown-like structure (CLS) quantification (M) from F4/80 (macrophage, red)-stained eWAT sections (N). Adipocytes were stained green for lipids (Bodipy) or perilipin 1 (PLIN1).

Scale bars, (K) 50 μm and (N) 100 μm. The number of mice is indicated in the figure. Data are represented as mean ± SEM. *p < 0.05, calculated with two-tailed unpaired t test.