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. 2000 May;123(1):111–124. doi: 10.1104/pp.123.1.111

Figure 2.

Figure 2

Figure 2

Immunolocalization of MIP-A, MIP-B, MIP-C, and MIP-F in immature roots of M. crystallinum. Fixed cross-sections (8–10 μm) of minor immature roots within 3 mm of the meristem were incubated with anti-MIP antibodies followed by goat anti-rabbit IgG coupled to Cy-5. Individual images for the emission of autofluorescence and for the Cy-5 fluorochrome were collected sequentially from the same optical section of the tissue, pseudocolored, and merged. Red/orange represents autofluorescence and green identifies MIP localization. Co-localization of the Cy-5 and tissue fluorescence is represented by yellow. The section shown in A and C was successively stained with MIP-A and MIP-B preimmune serum. A, C, E, and G represent images after staining with preimmune serum for MIP-A, MIP-B, MIP-C, and MIP-F, respectively. B, D, F, and H are stained with serum to MIP-A, MIP-B, MIP-C, and MIP-F, respectively. The bars represent 150 μm.