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. 2018 Apr 13;14(4):e1007294. doi: 10.1371/journal.pgen.1007294

Fig 1. PhyT (formerly PhyP) transfers phosphoryl groups from SdrG~P to PhyR.

Fig 1

In vitro phosphotransfer from SdrG~P to PhyT and further to PhyR in absence and presence of NepR over time. SdrG (10 μM) was phosphorylated using PakF (autophosphorylated with [γ-32P] ATP) on Ni-NTA columns. PhyR (5 μM), NepR (7.5 μM) and E. coli membrane particles (5 mg membrane fraction/mL) harboring either wild-type PhyT or the PhyT (H341A) derivative as a control were added as indicated. For confirmation of comparable amounts of PhyT and PhyT (H341A), Western blot analysis was conducted (S2A Fig).