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. 2018 Apr 13;14(4):e1007294. doi: 10.1371/journal.pgen.1007294

Fig 2. Phosphorylatable SdrG is essential for its positive regulatory role.

Fig 2

β-galactosidase activity of the EcfG-dependent nhaA2p-lacZ fusion in indicated S. melonis Fr1 mutant backgrounds upon overnight overexpression of sdrG and variants from the vanillate-inducible pVH vector with 250 μM vanillate. pVH was used as empty-vector control. Black bars and gray bars represent β-galactosidase activity pre- and 1 h post-induction with the chemical stress mixture (1% ethanol, 80 mM NaCl and 50 μM TBHP), respectively. Values are given as mean ±SD of three independent experiments.