Table 2. Definition, justification, interpretation of used biomarkers with zooplankton examples from literature.
Biomarker | Definition | Justification | Interpretation ↑ or ↓ |
Example of species and study area | Reference | |
---|---|---|---|---|---|---|
GSH: GSSG | Reduced: oxidised glutathione ratio | OD | Assays glutathione redox state [29] | Low ratio or increase in GGSG indicate stress | Limnocalanus macrurus, Baltic Sea, FI | [16] |
GR | Glutathione reductase | AO | Reduces GSSG back to GSH [30] | ↑ indicates more AO | L. macrurus, Baltic Sea, FI | [16] |
GST | Glutathione S-transferase | AO | Catalyzes reactions that detoxify harmful compounds [31] | ↑ indicates more AO | L. macrurus, Baltic Sea, FI; Eurytemora affinis, Seine estuary, FR | [16, 27] |
SOD | Superoxide dismutase | AO | Part of enzymatic defense to remove O2- [31] | ↑ indicates more AO | L. macrurus, Baltic Sea, FI | [16] |
CAT | Catalase | AO | Part of enzymatic defense to remove H2O2 [31] | ↑ indicates more AO | Boeckella gibbosa, Mixodiaptomus laciniatus, Lake Los Cantaros, ARG; Lake La Caldera, ESP | [32] |
ORAC | Oxygen reactive absorbance capacity | AO | Assesses antioxidant capacity [33] | ↑ indicates more AO | Acartia bifilosa, Baltic Sea, FI | [21] |
LPX | Lipid peroxidation | OD | Oxidative degradation of lipids in cell membrane, resulting in cell damage [33] | ↑ indicates stress | L. macrurus, Baltic Sea, FI | [16] |