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. 2018 Apr 3;14(4):e1006972. doi: 10.1371/journal.ppat.1006972

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© 2018 Vigneron et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A-D) Ultrastructure of cardia inf+/- near the PM secreting cells. (B) is a magnification of the black frame in (A) showing the PM (blue arrowhead). (C) and (D) two independent cardia organs showing the same region near PM secreting cells. Trypanosomes are observed packed within the ES near the location of PM secretion. At this point, several trypanosomes observed present vacuolation and nuclear condensation (orange arrowheads) indicative of cell death. Micrographs in this image represent three of five biological replicates analyzed. MG: midgut; FG: foregut; TR: trypanosomes; PM: peritrophic matrix; PM sc: PM secreting cells; mv: microvilli.