Fig 6. Mitochondrial integrity in cardia from inf+/+ and inf+/- tsetse.

(A) Effect of infection on mitochondria related gene expression. Heatmap generated from the fold-changes between control and either inf+/- or inf+/+ cardia. The * denote the level of significance associated with the DE of specific transcripts (*p<0.05; **p<0.01; ***p<0.001; ****p<0.0001). (B-D) Ultrastructure of the sphincter myofibrils in control non-inf (B), inf+/- (C) and inf+/+ (D) cardia. White arrows show the mitochondria, red arrowheads show patterns of mitochondria degradation, and yellow arrows show dilatation of sarcoplasmic reticulum. (E-G) Ultrastructure of giant lipid-containing cells in control non-inf (E), inf+/- (F) and inf+/+ (G) cardia. In both infection phenotypes, mitochondria cristae appear disogarnized compared to control. Micrographs in this image represent one of three, five and six of biological replicates from cardia non-inf, inf+/- and inf+/+, respectively. White arrows show the mitochondria. Ld, lipid droplets; Nu, nucleus.