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Distribution of GUS (monomeric Rs = approximately 3.3 nm) when injected alone (A) or co-injected with 3-kD F-dextran (B). In both experiments, GUS showed patchy unloading with no evidence of subsequent post-phloem movement. The distribution of co-injected 3-kD F-dextran (C) was almost entirely coincident with that of GUS (B). (The chalazal walls show some autofluorescence.) The section shown in A was partially cleared with 80% (v/v) ethanol. ch, Chalaza; nu, nucellus; x, xylem.
