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. Author manuscript; available in PMC: 2018 Apr 13.
Published in final edited form as: Sci Pages Pulmonol. 2017;1(1):7–18.

Figure 6.

Figure 6

Time-dependent decrease in trans-endothelial resistance (TER) of mouse pulmonary microvascular endothelial cells (A) LPS (0.3 μg/mL or 0.17 μg/mL) and (B) nocodazole (1 μM or 5 μM). Eight-well arrays were inoculated with mouse pulmonary microvascular endothelial cells (50,000); 48–72 h later, confluent monolayers were observed exhibiting TER of ≈800 MΩ to ≈900 MΩ. (A) LPS or; (B) Nocodazole or veh were added and TER values recorded continuously at 20-s intervals over the next 6 h. Data are representative of three separate experiments; (C) PCR analysis for TLR4 expression (Inset) RT-Polymerase chain reaction analysis for toll like receptor-4 (TLR4) expression in endothelial cell from murine lungs. GAPDH expression was used as a housekeeping control.