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. Author manuscript; available in PMC: 2018 Apr 13.
Published in final edited form as: Sci Pages Pulmonol. 2017;1(1):7–18.

Table 1.

Products and supplies for culture of mouse pulmonary microvascular endothelial cells.

Product Supplier Supplier address Product number
Dulbeccos’s modified Eagles’s medium (DMEM), powder, high glucose Thermo Fisher Scientific West Columbia, SC, USA 12100-046
Fetal bovine serum (FBS) Sigma-Aldrich St. Louis, MO, USA SV30014.03 or F0926
Endothelial basal medium-2 (EBM-2) and BulletKit™ Lonza Allendale, NJ, USA CC-3156
Penicillin and streptomycin Thermo Fisher Scientific As above 15140122
Fibronectin Thermo Fisher Scientific As above 33016015
Gelatin Sigma-Aldrich As above G1890
Collagen type I (PureCol) Advanced BioMatrix San Diego, CA, USA 5005-100 ML
Hank’s balanced salt solution (HBSS) Thermo Fisher Scientific As above 14185052
Phosphate-buffered saline (PBS) without Ca2+ and Mg2+ Thermo Fisher Scientific As above 14190250
Bovine serum albumin (BSA) Thermo Fisher Scientific As above A9647
Collagenase type I Worthington Biochemical Lakewood, NJ, USA LS004196
Dynabeads® sheep anti-rat IgG Thermo Fisher Scientific As above 11035
DynaMag® spin magnet (MPC) Thermo Fisher Scientific As above 12320D
Platelet endothelial cell adhesion molecule (PECAM-1; CD31) *discontinued at TFS
May consider other company
Santa Cruz Santa Cruz, CA, USA Sc-376764
Cell strainer (70 μm) Thermo Fisher Scientific As above 22-363-548
Sterilization filter (0.22 μm) Thomas Scientific Swedesboro, NJ, USA 1226S77
0.05% Trypsin-EDTA Thermo Fisher Scientific As above 25300-054
Accutase Innovative Cell Technologies San Diego, CA, USA #AT-104
ECIS array Applied BioPhysics Troy, NY, USA 8W1E PC