Table 1.
Product | Supplier | Supplier address | Product number |
---|---|---|---|
Dulbeccos’s modified Eagles’s medium (DMEM), powder, high glucose | Thermo Fisher Scientific | West Columbia, SC, USA | 12100-046 |
Fetal bovine serum (FBS) | Sigma-Aldrich | St. Louis, MO, USA | SV30014.03 or F0926 |
Endothelial basal medium-2 (EBM-2) and BulletKit™ | Lonza | Allendale, NJ, USA | CC-3156 |
Penicillin and streptomycin | Thermo Fisher Scientific | As above | 15140122 |
Fibronectin | Thermo Fisher Scientific | As above | 33016015 |
Gelatin | Sigma-Aldrich | As above | G1890 |
Collagen type I (PureCol) | Advanced BioMatrix | San Diego, CA, USA | 5005-100 ML |
Hank’s balanced salt solution (HBSS) | Thermo Fisher Scientific | As above | 14185052 |
Phosphate-buffered saline (PBS) without Ca2+ and Mg2+ | Thermo Fisher Scientific | As above | 14190250 |
Bovine serum albumin (BSA) | Thermo Fisher Scientific | As above | A9647 |
Collagenase type I | Worthington Biochemical | Lakewood, NJ, USA | LS004196 |
Dynabeads® sheep anti-rat IgG | Thermo Fisher Scientific | As above | 11035 |
DynaMag® spin magnet (MPC) | Thermo Fisher Scientific | As above | 12320D |
Platelet endothelial cell adhesion molecule (PECAM-1; CD31) *discontinued at TFS May consider other company |
Santa Cruz | Santa Cruz, CA, USA | Sc-376764 |
Cell strainer (70 μm) | Thermo Fisher Scientific | As above | 22-363-548 |
Sterilization filter (0.22 μm) | Thomas Scientific | Swedesboro, NJ, USA | 1226S77 |
0.05% Trypsin-EDTA | Thermo Fisher Scientific | As above | 25300-054 |
Accutase | Innovative Cell Technologies | San Diego, CA, USA | #AT-104 |
ECIS array | Applied BioPhysics | Troy, NY, USA | 8W1E PC |