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A) Scheme of general neddylation tri-enzyme cascades. B) Cartoon of DCN1/RBX1-stimulated transfer of NEDD8 (“N8” in cartoon) from UBE2M to a cullin (CUL1), which can be monitored in vitro using a pulse-chase assay. Desired inhibitors will block transfer of Nedd8 C) TR-FRET assay for DCN1 binding to UBE2MNAc. Desired inhibitors will possess low FRET signal.
