Table 2.
FLIP7 and antibiotics interaction on planktonic cells (TTC assay)
| Strain/antibiotic | Antibiotic MIC90 (µg/mL) | Antibiotic MIC90 in FLIP7 presence (µg/mL)1 | FICI | Interaction type | Fold reduction2 |
|---|---|---|---|---|---|
| Staphylococcus aureus | |||||
| Meropenem | 0.02 ± 0.02 | <0.002 | 0.667 | Add | >10 |
| Amikacin | 1.88 ± 0.00 | 0.36 ± 0.12 | 0.833 | Add | 5.2 |
| Kanamycin | 3.51 ± 1.17 | <0.1 | 0.527 | Add | >35.1 |
| Ampicillin | 0.03 ± 0.01 | 0.007 ± 0.001 | 0.542 | Add | 4.3 |
| Vancomycin | 0.41 ± 0.19 | 0.12 ± 0.00 | 0.589 | Add | 3.4 |
| Benzalkonium Cl | 0.30 ± 0.00 | 0.15 ± 0.00 | 0.750 | Add | 2.0 |
| Clindamycin | 75.0 ± 0.0 | 4.70 ± 0.00 | 0.729 | Add | 15.9 |
| Erythromycin | 0.94 ± 0.00 | 0.03 ± 0.00 | 0.589 | Add | 31.3 |
| Oxacillin | 0.05 ± 0.00 | 0.014 ± 0.002 | 1.042 | Ind | 3.6 |
| Chloramphenicol | 4.70 ± 0.00 | 0.60 ± 0.00 | 0.418 | Syn | 7.8 |
| Escherichia coli | |||||
| Meropenem | 0.03 ± 0.00 | 0.03 ± 0.00 | 1.085 | Ind | 1.0 |
| Cefotaxime | 0.09 ± 0.00 | 0.02 ± 0.00 | 0.415 | Syn | 4.5 |
| Polymyxin B | 0.34 ± 0.11 | <0.02 | 0.339 | Syn | 17.0 |
| Ciprofloxacin | 0.03 ± 0.00 | 0.015 ± 0.00 | 0.666 | Add | 2.0 |
| Tetracycline | 0.46 ± 0.00 | 0.03 ± 0.00 | 0.729 | Add | 15.3 |
| Gentamicin | 4.69 ± 0.00 | 3.53 ± 1.17 | 1.085 | Ind | 1.3 |
| Chloramphenicol | 4.69 ± 0.00 | 0.30 ± 0.00 | 0.670 | Add | 15.6 |
| Pseudomonas aeruginosa | |||||
| Meropenem | 0.94 ± 0.31 | 0.48 ± 0.00 | 0.516 | Add | 2.0 |
| Klebsiella pneumoniae | |||||
| Meropenem | 0.01 ± 0.00 | 0.01 ± 0.00 | 1.042 | Ind | 1.0 |
| Acinetobacter baumannii | |||||
| Meropenem | 0.88 ± 0.13 | 0.03 ± 0.01 | 0.393 | Syn | 29.3 |
Notes:
1
MIC90 of antibiotic in combination with FLIP7 concentration causing maximum antibiotic fold reduction.
2
Ratio of antibiotic MIC90 in the absence and presence of FLIP7 concentration causing maximum antibiotic fold reduction. Data presented as mean ± SE.
Abbreviations: TTC, trimethyl tetrazolium chloride; MIC, minimum inhibiting concentration; FICI, fractional inhibitory concentration index; Syn, synergy; Add, additivity; Ind, independence.