Figure 2.

NKA α1 expression vector and siRNA altered its activity and subcellular expression. To overexpress or inhibit NKA α1 expression, the NKA α1 expression vector or siRNA was added to PTECs for 30 h before 48 h of UA 100 μg ml⁻¹ stimulation. The NKA α1 expression vector enhanced its activity and cell membrane expression, whereas its siRNA reduced its activity (A, B). We used Lyso-Tracker Red to label the lysosomes of cells (red) and then fixed the cells before proceeding to immunohistochemistry staining of NKA α1 (green). Confocal microscopy immunofluorescence showed that, under normal circumstances, NKA α1 was linearly expressed along the cell membrane (C–c). After adding UA, NKA α1 translocated into the cell plasma and were scattered into the lysosomes (C–f). The NKA α1expression vector increased its cell membrane expression and decreased its lysosome expression (C–i), whereas NKA α1 siRNA decreased its cell membrane expression and increased its lysosome expression (C–l). *P<0.05 vs Cont, **P<0.01 vs Cont, #P<0.05 vs UA, ##P<0.01 vs UA.